What is the difference between superscript ii and iii

Data are the average of three separate experiments. Highly structured or GC-rich transcripts can be difficult to reverse transcribe due to premature termination, resulting in truncated cDNAs. This represents a 4. Figure 4. Reverse transcription of a challenging GC-rich transcript. Panel A. Reactions were analyzed by agarose gel electrophoresis and ethidium bromide staining. Lane 1. No-template control. Lane 2. No-reverse transcriptase control. Lane 3. Lane 4. Panel B. Data are the average of three independent experiments.

Several key performance variables were tested: sensitivity of transcript detection, transcript length and sensitivity to a common RNA contaminant, ethanol. We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms.

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Explore Support Center Medical Affairs We provide medical information and facilitate research collaborations. Connect With Us. Local Sales Support Get in touch with a nearby distributor or sales representative. Find Sales Contact. Contact Us Customer Support. Abstract Accurate gene expression analysis depends on the reverse transcriptase used as well as other factors e.

Transcript Sensitivity Gene expression analysis is the most common use for RTs. Ethanol Sensitivity Ethanol is routinely used in nucleic acid purification protocols.

Difficult Transcript Highly structured or GC-rich transcripts can be difficult to reverse transcribe due to premature termination, resulting in truncated cDNAs. Reagents were provided by Promega Corporation.

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Connect With Us. Local Sales Support Get in touch with a nearby distributor or sales representative. Find Sales Contact. Contact Us Customer Support. Abstract Different reverse transcriptases have different characteristics, and some are more well suited to specific applications than others.

References Temin, H. Nature , —3. Baltimore, D. Nature , — Krug, M. Methods Enzymol. Brooks, E. Biotechniques 19 , — Gerard, G. Nucleic Acids Res. DeStefano, J.

Biol Chem. Harrison, G. Kotewicz, M. Sellner, L. Ahokas, H. PCR Methods Appl. Tanese, N. USA 85 , — Focus 16 , —3. GoScript is a trademark of Promega Corporation. SuperScript is a registered trademark of Life Technologies Corporation. Related Resources. Reverse Transcriptase Comparison Study This article compares 5 popular reverse transcriptases to determine which offers superior performance. Americas Brazil. United States. Pacific Asia Australia. Korea, Republic of.